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The present invention relates to a kind of drug-film affinity assay method take poly-diacetylene Optochemical sensor as the basis, belong to the Pharmaceutical Analysis technical field. Cell membrane is important drug target, also be the physiologic barrier that medicine enters cell simultaneously, active and the transhipment in human body and the drug-film affinity of medicine are closely related, and therefore at the new drug development initial stage cell membrane affinity of medicine being carried out in-vitro screening is an extremely important research contents.

The liposome model that employing has the double-layer of lipoid structure comes analog cell film and actual conditions then more approaching. In recent years, some occur and measured the new technology of drug-film affinity with the lipid membrane model. Also have researcher’s application surface plasma resonance sensing technology SPR to measure drug-film affinity abroad, advanced but this technical equipment requires, testing cost is high, and most domestic medicament research and development mechanism is difficult to bear.

Some other method for example fixed die carrier technology SSLM and pH titration rule is not suitable for the rapid screening of drug-film affinity because analytic process is complicated. Therefore, be to improve new drug development speed and the scale of China, need a kind of easy, quick, favorable reproducibility and the low drug-film affinity assay method of testing cost badly.

The present invention is directed to deficiency and the defective of existing drug-film affinity assay method, with cheaply, be used for drug-film affinity based on poly-diacetylene PDA the vesica sensing detection technology of photochemistry signal transport properties and measure, for new drug development unit and mechanism provide a kind of easy, quick, favorable reproducibility and the low drug-film affinity assay method of testing cost.

When poly-diacetylene sensor surface bioactive molecule generation molecular recognition reaction, poly-diacetylene skeleton is subjected to stir and causes conformation change, and color changes ruddiness into from blueness, and this recognition reaction is very responsive, can observe directly by naked eyes. Poly-diacetylene sensor can resemble the thin film sensor that consists of two dimension other sensor, and the outstanding feature that is different from other optical sensor is: poly-diacetylene is amphipathic molecule, in solution, can automatically form the poly-diacetylene vesica with double membrane structure, consist of the vesica sensor, the three-dimensional effect that forms behind the surface insertion bioactive molecule is closer to actual physiological environment.

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